E2F1 Knockout cell line(PC-3)

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E2F1 Knockout cell line(PC-3) Order

E2F1 Knockout cell line(PC-3)
Catalog#
YKO-HJ118
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
E2F1 Knockout cell line(PC-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
E2F1 Knockout cell line (PC-3)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
E2F1
Gene ID
1869
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%F12K+10%FBS
Target region
Knockout region: Exon 3 (based on transcript E2F1-201)
Validation: PCR+Sanger Sequencing
E2F1 Knockout cell line(PC-3) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:PC-3		 STR Info (Cell bank)
Cell Line:PC-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11111111
D7S820 811811
D16S539 11111111
VWA 17171717
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the E2F1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the PC-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, E2F1 gene knockout E2F1 Knockout cell line (PC-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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